Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X, for Western Blot Washing) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Sigma Aldrich Fine Chemicals Biosciences Trizma base solution | 77-86-1 | MFCD00004679 | 100 mL
Trizma base solution | Mol Wt: 121.135 | 77-86-1 | MFCD00004679 | 100 mL
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 1L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Boston Bioproducts Inc 10mM Tris-HCl pH 8.5 1L
Tris-HCl Buffer (10 mM pH 8 5) - 1L (For Research Use and Further Manufacturing Only)
Quality Biological Inc TE, pH 7.4 100mL
TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.
Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immuno-histochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Medchemexpress LLC NVP-TAE 684 | 761439-42-3 | 99.7% | 614.20 g/mol | C30H40ClN7O3S | 100 MG
NVP-TAE 684 is a potent, selective anaplastic lymphoma kinase (ALK) inhibitor used in research to block proliferation of ALCL-derived and ALK-dependent cell lines. It shows low-nanomolar activity and is supplied as a high-purity small molecule for in vitro studies and compound screening.
- Potent ALK inhibition with IC50 in the low-nanomolar range (≈2-10 nM).
- Selective activity against ALK-dependent and ALCL-derived cell lines.
- Suitable for in vitro research and compound screening applications.
- Available as solid and DMSO solution formats and multiple pack sizes.
- High purity suitable for research use (>99.7%).
Alkali Scientific CellPro™ Tris Glycine SDS 10X, pH 8.3, 500 mL
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Bioworld TBS Buffer, Low Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.