Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld Tris-Triton Buffer, 500 mL
Tris-Triton bufferis the idealbuffer with detergent required to release membrane- or cytoskeleton- bound proteins.Contains: 10mM Tris (pH 7.4), 100mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 10% Glycerol and 0.1% SDSAppearance: Liquid
Teknova Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Biotang Inc HEPES Protein Extraction Buffer (5X), 250 ml
HEPES Protein Extraction Buffer (5X), 250 ml.
Boston Bioproducts Inc Bis-Tris Buffer (0.2 M, pH 6.5) - 250ml
The Bis-Tris Buffer is a zwitterionic agent commonly used in biological applications. It maintains a pH within 5.8 to 7.2, ideal for biological processes that require stable environments within this range. It is particularly effective in applications such as protein and nucleic acid purification, biochemical assays, and cell culture. The buffering capacity of Bis-Tris is influenced by its concentration, while its pH can be adjusted based on the ratio of Bis-Tris to its conjugate acid or base.
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eMolecules 895245-31-5 | trans-3,4-Bis-hydroxymethyl-pyrrolidine-1-carboxylic acid tert-butyl ester | J&W PharmLab LLC462.584 | C22H42N2O8 | 97.000 | CC(C)(C)OC(=O)N1C[C@H](CO)[C@@H](CO)C1.CC(C)(C)OC(=O)N1C[C@@H](CO)[C@H](CO)C1 | 1g | 592901059
trans-3,4-Bis-hydroxymethyl-pyrrolidine-1-carboxylic acid tert-butyl ester | J&W PharmLab LLC | 895245-31-5462.584 | C22H42N2O8 | 97.000 | CC(C)(C)OC(=O)N1C[C@H](CO)[C@@H](CO)C1.CC(C)(C)OC(=O)N1C[C@@H](CO)[C@H](CO)C1 | 1g | 592901059
Selleck Chemical LLC P62-mediated mitophagy inducer
P62-mediated mitophagy inducer (PMI) is a P62-mediated activator of mitophagy that functions independently of parkin recruitment or mitochondrial membrane potential ( m) collapse It remains effective even in cells lacking a fully functional PINK1/Parkin pathway PMI stabilizes Nrf2 and enhances p62 expression thereby activating mitophagy
Selleck Chemical LLC Trometamol S4176-50mg
Trometamol(Tromethamine) is a proton acceptor used to treat acidemia
Medchemexpress LLC P62-mediated mitopha 50mg | 1809031-84-2 | 392.15 | C14H9IN4O2 | 50 MG
P62-mediated mitophagy inducer is a small-molecule activator of p62-dependent mitophagy used in research to stimulate mitochondrial autophagy without recruiting Parkin or collapsing mitochondrial membrane potential.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Promotes mitochondrial autophagy while preserving mitochondrial membrane potential.
- Suitable for autophagy and mitophagy research applications.
- Powder form with defined cold-storage stability for long-term storage.
- Available in small research pack sizes for laboratory use.
- Molecular formula C14H9IN4O2; molecular weight 392.15 g·mol⁻¹.
Boston Bioproducts Inc 10mM Tris-HCl pH 8 1L
Tris-HCl Buffer (10 mM pH 8 0) - 1L (For Research Use and Further Manufacturing Only)
Medchemexpress LLC Nvp-tae 226 | 761437-28-9 | MFCD12031516 | 99.8% | C23H25ClN6O3 | 10MG
NVP-TAE 226 is a potent, ATP-competitive small-molecule inhibitor of focal adhesion kinase (FAK) and insulin-like growth factor 1 receptor (IGF-1R), used in preclinical oncology and signal-transduction research. It exhibits activity across multiple kinases and has demonstrated antitumor effects in vitro and in vivo.
- Potent FAK inhibition (IC50 5.5 nM).
- Dual activity against IGF-1R and Pyk2 (IC50 140 nM and 3.5 nM).
- Additional activity against InsR, c-Met, KDR, and Flt3.
- High purity suitable for research (~99.8%).
- Stable as powder under recommended storage conditions.